by Jessica Helweg | Sep 16, 2019
Refer to the package insert for each product. If you have further questions, contact VitaCyte technical support.
by Jessica Helweg | Sep 16, 2019
Refer to the package insert for each product. If you have further questions, contact VitaCyte technical support.
by Jessica Helweg | Sep 16, 2019
Refer to the package insert for each product. If you have further questions, contact VitaCyte technical support.
by Jessica Helweg | Sep 16, 2019
Product abbreviations: Collagenase HA = purified collagenase with high collagen degradation activity (CDA) Collagenase MA = purified collagenase with moderate CDA (about 50% of Collagenase HA) BP Protease = neutral protease from Bacillus polymyxa (DispaseTM Equivalent...
by Jessica Helweg | Sep 16, 2019
There are over 200 mature cell types in mammals. A limited number of these cells are routinely used for research studies. These include islets and acinar cells from the pancreas, hepatocytes and non-parenchymal cells from the liver, cardiomyocytes, neural cells, and...
by Jessica Helweg | Sep 16, 2019
Application Specific Formulations are pre-blended mixtures of purified collagenase and protease in optimal ratios for isolation of cells from a particular tissue and/or species. These formulations provide a recommended dose for use with an associated method. These...
by Jessica Helweg | Sep 16, 2019
This is dependent on the cell you are isolating. VitaCyte has enzyme product/formulations and recommended doses to isolate: Rodent Islets Porcine Islets Non-Human Primate Islets Human Islets Rodent and Human Hepatocytes Rodent Cardiomyocytes Human Adipose These...
by Jessica Helweg | Sep 16, 2019
It is difficult to come up with a reliable conversion factors to translate enzyme activities from one supplier to those from another supplier. Some of the factors that may affect this value are differences in the assay precision by one or both suppliers, differences...
by Jessica Helweg | Sep 16, 2019
The PD Collagenase, Collagenase Gold, Collagenase Gold Plus, BP Protease, and CIzyme AS products are stable for several days at ambient temperatures. For other products, contact VitaCyte technical support.
by Jessica Helweg | Sep 16, 2019
To convert DMC units to FITC-BSA units, multiply the DMC units by 13,400. For example, if you are currently using 1.75 DMC U per gram then you would target 23,450 FITC-BSA units for equivalent activities.
by Jessica Helweg | Sep 16, 2019
The DispaseTM II product is a cruder preparation of neutral protease than our CIzyme™ BP protease, but both originate from Paenibacillus polymyxa fermentations . The FITC-BSA specific activity of the Dispase II product is less than 10% that of our...
by Jessica Helweg | Sep 16, 2019
To be determined. VitaCyte’s products are labeled for research use only or as GMP Grade. All products are manufactured in accordance with the principles for clinical trial material outlined in the International Conference on Harmonization (ICH) document ICH Q7A, ...
